Interaction Details


Details of experiments between K9N5R3 and Q8TF65
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV

Protein B

Protein: GIPC2
Fragment: 112-203
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.24
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.21
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV

Protein B

Protein: GIPC2
Fragment: 112-203
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MERS-E
Fragment: 73-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: Biotin-ado-ado-KPPLPPDEWV

Protein B

Protein: GIPC2
Fragment: 112-203
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.1
Normalized holdup BI: -0.1
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6