Interaction Details


Details of experiments between K9N5R3 and P50479
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV

Protein B

Protein: PDLIM4
Fragment: 1-83
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 3.83
PUBMED ID: 36115835