Interaction Details


Details of experiments between A6NIM6 and Q6P0Q8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0.04
Standard deviation of dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 7.41
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.63
Immobilized partner concentration (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 6.25
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.81
Normalized holdup BI: 0.9
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 6.06
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 4

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.89
Normalized holdup BI: 0.89
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 6.03
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 5

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: Biotin-ado-ado-SSIDLWETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.92
Normalized holdup BI: 0.92
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.94
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.7
Holdup BI standard deviation: 0.21
Normalized holdup BI: 0.7
Normalized holdup BI standard deviation: 0.21
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.44
PUBMED ID: 36115835