Interaction Details


Details of experiments between A6NIM6 and Q14160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.54
Immobilized partner concentration (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.93
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.78
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.82
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 5
Measured pKd: 5.76
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 1.94
Standard deviation of dissociation constant (10-6M): 0.13
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.71
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.72
Holdup BI standard deviation: 0.07
Normalized holdup BI: 0.8
Normalized holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.71
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.7
Normalized holdup BI: 0.77
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.63
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 6

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.62
Normalized holdup BI: 0.77
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.62
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 7

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.42
Immobilized partner concentration (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.59
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.61
Normalized holdup BI: 0.73
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.53
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 9

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: Biotin-ado-ado-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.8
Normalized holdup BI: 0.8
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.44
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 10

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: Biotin-ado-ado-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.68
Normalized holdup BI: 0.8
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.42
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 11

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.44
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.55
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 5.14
PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.38
Normalized holdup BI: 0.5
Immobilized partner concentration (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.05
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 13

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 1090-1197
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.97
PUBMED ID: 36115835

Experiment series 14

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: biotin-ttds-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.81
PUBMED ID: 36115835

Experiment series 15

Protein A

Protein: SLC15A5
Fragment: 570-579
Site: PBM
Sequence: SSIDLWETAL
Construct: Biotin-ado-ado-SSIDLWETAL

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.5
Normalized holdup BI: 0.5
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.79
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6