Interaction Details

Details of experiments between A6NHR9 and P57105
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: SMCHD1
Fragment: 1996-2005
Site: PBM
Sequence: QNRIITKTDV
Construct: biotin-ado-ado-QNRIITKTDV

Protein B

Protein: SYNJ2BP
Fragment: 6-103
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.22

Normalized holdup BI: 0.23

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.24

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.