Interaction Details


Details of experiments between A4U7F2 and Q6P0Q8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.78
Normalized holdup BI: 0.78
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 22
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.27
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 11.01
Standard deviation of dissociation constant (10-6M): 1.24
Average holdup BI: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 0
P value: 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.96
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.41
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 10
P value: 0
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.91
PUBMED ID: 36115835