Interaction Details


Details of experiments between A4U7F2 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.56
Normalized holdup BI: 0.56
Immobilized partner concentration (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.81
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV

Protein B

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.4
Normalized holdup BI: 0.4
Immobilized partner concentration (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.52
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV

Protein B

Protein: DLG2
Fragment: 189-283
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.46
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV

Protein B

Protein: DLG2
Fragment: 93-186
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.25
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV

Protein B

Protein: DLG2
Fragment: 418-513
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.2
PUBMED ID: 36115835