Interaction Details


Details of experiments between A4D2P6 and Q9P2M7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRID2IP
Fragment: 272-370
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CGN
Fragment: 1188-1197
Site: PBM
Sequence: TESNLQTSSC
Construct: Biotin-Ahx-TESNLQTSSC
Average holdup BI: 0.09
Normalized holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.8
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: GRID2IP
Fragment: 1-87
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CGN
Fragment: 1188-1197
Site: PBM
Sequence: TESNLQTSSC
Construct: Biotin-Ahx-TESNLQTSSC
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835