Interaction Details


Details of experiments between A4D2P6 and Q9NS75
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRID2IP
Fragment: 272-370
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: Biotin-ado-ado-SVWLRKETRV
Average holdup BI: 0.09
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.73
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: GRID2IP
Fragment: 272-370
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV
Average holdup BI: 0.06
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.07
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.45
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: GRID2IP
Fragment: 1-87
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV
Average holdup BI: -0.15
Normalized holdup BI: -0.15
Immobilized partner concentration (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: GRID2IP
Fragment: 1-87
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV
Average holdup BI: -0.11
Immobilized partner concentration (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: GRID2IP
Fragment: 1-87
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: Biotin-ado-ado-SVWLRKETRV
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: GRID2IP
Fragment: 272-370
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CYSLTR2
Fragment: 337-346
Site: PBM
Sequence: SVWLRKETRV
Construct: biotin-ttds-SVWLRKETRV
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835