Interaction Details


Details of experiments between A4D2P6 and Q96KB5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRID2IP
Fragment: 272-370
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PBK
Fragment: 313-322
Site: PBM
Sequence: HIVEALETDV
Construct: biotin-ttds-HIVEALETDV
Average holdup BI: 0.28
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.95
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: GRID2IP
Fragment: 272-370
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PBK
Fragment: 313-322
Site: PBM
Sequence: HIVEALETDV
Construct: biotin-ttds-HIVEALETDV
Average holdup BI: 0.08
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 11.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.96
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: GRID2IP
Fragment: 1-87
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PBK
Fragment: 313-322
Site: PBM
Sequence: HIVEALETDV
Construct: biotin-ttds-HIVEALETDV
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: GRID2IP
Fragment: 1-87
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PBK
Fragment: 313-322
Site: PBM
Sequence: HIVEALETDV
Construct: biotin-ttds-HIVEALETDV
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 11.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835