Interaction Details


Details of experiments between A4D2P6 and Q82005
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRID2IP
Fragment: 272-370
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV73-E6
Fragment: 139-148
Site: PBM
Sequence: SGSRPSATVV
Construct: biotin-ttds-SGSRPSATVV
Average holdup BI: 0.28
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.64
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: GRID2IP
Fragment: 1-87
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV73-E6
Fragment: 139-148
Site: PBM
Sequence: SGSRPSATVV
Construct: biotin-ttds-SGSRPSATVV
Average holdup BI: -0.16
Normalized holdup BI: -0.16
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: GRID2IP
Fragment: 1-87
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV73-E6
Fragment: 139-148
Site: PBM
Sequence: SGSRPSATVV
Construct: biotin-ttds-SGSRPSATVV
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: GRID2IP
Fragment: 272-370
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV73-E6
Fragment: 139-148
Site: PBM
Sequence: SGSRPSATVV
Construct: biotin-ttds-SGSRPSATVV
Average holdup BI: 0.01
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835