Interaction Details

Details of experiments between A1X283 and P56282
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: SH3PXD2B
Fragment: 1-911
Construct: endogenous proteins (Jurkat)

Protein B

Protein: POLE2
Fragment: 1-527
Construct: avi-his6-MBP-fusion protein

Average holdup BI: 0.18

Immobilized partner concentration (10^-6M): 26

P value: 0.45

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic equation

Date of measurement: 2024-02-02

Number of measurements: 6

The affinity was below the detection threshold of the assay.

Experimental Details

Batch = Helsinki_batch6; Ncontrols = 12; Nsamples = 6; Npreys = 6515; threshold curvature: 2.18