Interaction Details

Details of experiments between A0A075B767 and Q12959
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PPIAL4H
Fragment: 1-164
Construct: endogenous protein (Jurkat)

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Average holdup BI: 0.2

Immobilized partner concentration (10^-6M): 10

P value: 0.92

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic binding equation

Date of measurement: 2022-08-03

Number of measurements: 3

The affinity was below the detection threshold of the assay.

PUBMED ID: 36542723

Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2